How to use Kjeldahl Nitrogen Analyzer


Time of issue:

2021-09-15

The chemical decomposition process of the Kjeldahl Nitrogen Analyzer 1. When digesting with Kjeldahl Nitrogen Analyzer, 6 Kjeldahl flasks should be prepared and labeled. Add 1.0 mL of protein solution of appropriate concentration to flasks 1, 2, and 3 respectively. The sample should be added to the bottom of the flask, and do not stick to the mouth and neck of the flask.

The chemical decomposition process of the Kjeldahl Nitrogen Analyzer
1. When digesting with Kjeldahl Nitrogen Analyzer, 6 Kjeldahl flasks should be prepared and labeled. Add 1.0 mL of protein solution of appropriate concentration to flasks 1, 2, and 3 respectively. The sample should be added to the bottom of the flask, and do not stick to the mouth and neck of the flask.

Kjeldahl Nitrogen Analyzer components

In the Kjeldahl Nitrogen Analyzer, 0.3 g of potassium sulfate-copper sulfate contact agent, 2.0 mL of concentrated sulfuric acid, and 1.0 mL of 30% hydrogen peroxide were added in sequence. Flasks 4, 5, and 6 are used as blank controls to determine trace nitrogen-containing substances that may be contained in the reagents, and to calibrate the sample determination. 4, 5, and 6 Kjeldahl Nitrogen Analyzer flasks were added with 1.0 mL of distilled water instead of the sample solution.

2. Place the flasks of each Kjeldahl Nitrogen Analyzer with reagents on the chemical decomposition rack, and connect the air extraction device. First, boil it with low heat. At this time, the contents of the flask of the Kjeldahl Nitrogen Analyzer are carbonized and blackened, and a large amount of foam is generated. Be careful to prevent the bubbles from rushing out of the nozzle.

After the bubbles in the flask of the Kjeldahl Nitrogen Analyzer disappear and stop producing, increase the firepower to keep the liquid in the bottle slightly boiling. After the solution in the Kjeldahl Nitrogen Analyzer's flask was clarified, the heating was continued to make the chemical decomposition solution slightly boil for 15 minutes. During the digestion process of the Kjeldahl Nitrogen Analyzer, the flask should be rotated at any time so that all the sticky substances on the inner wall can flow to the bottom to ensure the complete digestion of the sample. The gas released during digestion contains SO2, which is strongly irritating, so the pump should be turned on from beginning to end to pump the gas into tap water for discharge. The entire Kjeldahl Nitrogen Analyzer digestion process should be performed in a fume hood. After digestion is complete, turn off the flame and allow the flask to cool to room temperature.

Through the above introduction and analysis of the Kjeldahl Nitrogen Analyzer, hope it helps you.