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The chemical decomposition process of the Kjeldahl Nitrogen Analyzer
Time of issue:
2021-09-15
The chemical decomposition process of the Kjeldahl Nitrogen Analyzer. When using the instrument for digestion, prepare and label 6 Kjeldahl flasks. Add 1.0 mL of protein solution of appropriate concentration to flasks 1, 2, and 3, respectively. The sample should be added to the bottom of the vial without sticking to the mouth and neck of the vial.
The chemical decomposition process of the Kjeldahl Nitrogen Analyzer. When using the instrument for digestion, prepare and label 6 Kjeldahl flasks. Add 1.0 mL of protein solution of appropriate concentration to flasks 1, 2, and 3, respectively. The sample should be added to the bottom of the vial without sticking to the mouth and neck of the vial.
In the Kjeldahl Nitrogen Analyzer, 0.3 g of potassium sulfate-copper sulfate contact agent, 2.0 mL of concentrated sulfuric acid, and 1.0 mL of 30% hydrogen peroxide were added sequentially. Vials 4, 5, and 6 are used as blanks to determine trace nitrogenous species that may be present in reagents and to calibrate sample assays. Add 1.0 mL of distilled water in place of the sample solution to 4, 5, and 6 Kjeldahl nitrogen cylinders.
Place each flask containing reagents on a chemical disintegration rack and connect an aspirator. First, bring to a boil over low heat. At this point, the contents of the Kjeldahl Nitrogen Analyzer's flask were charred and black, and a lot of foam was generated. Be careful to prevent air bubbles from rushing out of the nozzle.
After the bubbles in the flask of the Kjeldahl Nitrogen Analyzer disappeared and stopped generating, increase the firepower to keep the liquid in the flask slightly boiling. After the solution in the Kjeldahl nitrogen analysis flask was clear, heating was continued to slightly boil the chemical decomposition solution for 15 minutes.
During the digestion process, the flask must be rotated at any time so that all the adhesive on the inner wall flows to the bottom to ensure complete digestion of the sample. The gas released during the digestion process contains SO2 and is highly irritating, so the pump must be run from start to finish, pumping the gas into the tap water for discharge. The entire Kjeldahl digestion process should be performed in a fume hood. When digestion is complete, turn off the flame and allow the flask to cool to room temperature.
Through the above introduction and analysis of the chemical decomposition process of the Kjeldahl Nitrogen Analyzer, hope it helps you.
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