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Introduction of the Kjeldahl nitrogen analyzer
Time of issue:
2021-09-15
The Kjeldahl nitrogen analyzer is an instrument that calculates the protein content by measuring the nitrogen content in the sample based on the principle that the nitrogen content in the protein is constant. Because the method of protein content measurement and calculation is called the Kjeldahl nitrogen determination method, it is called Kjeldahl nitrogen determination instrument, also known as nitrogen determination instrument, protein determination instrument, or crude protein determination instrument.
The Kjeldahl nitrogen analyzer is an instrument that calculates the protein content by measuring the nitrogen content in the sample based on the principle that the nitrogen content in the protein is constant. Because the method of protein content measurement and calculation is called the Kjeldahl nitrogen determination method, it is called Kjeldahl nitrogen determination instrument, also known as nitrogen determination instrument, protein determination instrument, or crude protein determination instrument.
principle. The principle of the Kjeldahl nitrogen analyzer is to convert the nitrogen into ammonium sulfate by co-heating organic compounds with sulfuric acid. During this step, potassium sulfate is often added to the mixture to raise the boiling point of the intermediate product. The endpoint of the analytical process of the sample is well judged because the mixture becomes colorless and transparent (dark at first) at this point.
A small amount of sodium hydroxide was added to the resulting solution, followed by distillation. In this step, the Kjeldahl analyzer will convert the ammonium salts into ammonia. The total ammonia content (which is directly determined by the nitrogen content of the sample) is determined by back titration: the end of the condenser tube is immersed in a solution of boric acid. Ammonia will react with acid, and excess acid will be titrated with sodium carbonate under the direction of methyl orange. The result obtained from the titration is multiplied by a specific conversion factor.
Scope of application. The kjeldahl instrument is a Kjeldahl method to detect ammonia, protein nitrogen content, phenol, volatile fatty acids, cyanide, sulfur dioxide, ethanol, etc. in grains, food, feed, water, soil, sludge, sediment, and chemicals content. It has a very good price/performance ratio, and only manual operation is required for the titration process, which is very suitable for routine testing in laboratories and inspection institutions. It is widely used for nitrogen or protein content analysis of food, crops, seeds, soil, fertilizers, and other samples.
Through the above introduction and analysis of the Kjeldahl nitrogen analyzer, hope it helps you.
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